Journal: ACS nano

Article Title: Echinacoside-Zinc Nanomaterial Inhibits Skin Glycation by Suppressing the Transcriptional Activation of the Receptor for Advanced Glycation End-Products.

doi: 10.1021/acsnano.3c04726

Figure Lengend Snippet: Figure 2. PPZn exerted anti-antiglycan in glycated model mouse skin. (A−C) Representative images of immunohistochemical (IHC) staining (A−a), HE staining (B−a), and Masson staining (C−a) of mouse skin tissue after the indicated treatment. The statistical results of the IHC staining index (A−b), epidermal thickness (B−b), and collagen density (C−b) are shown on the right. Scale bar, 50 μm. (D) qRT- PCR detection of RAGE mRNA levels in skin tissues of mice after the indicated treatment. (E) Western blot representative images and quantitative analysis of RAGE, COL1A2, MMP1, AGEs, and β-actin protein levels in skin tissues of mice after the indicated treatment. (F) Representative images of TUNEL staining of mouse skin tissue after the indicated treatment. Scale bar, 50 μm. (G−I) Relative Hyp content (G), SOD activity (H), and MDA concentration (I) in skin tissues of mice after the indicated treatment. All values are presented as the mean ± SD; P-values determined by two-sided Student’s t test. *P < 0.05, **P < 0.01, compared with the model.

Article Snippet: After being blocked, samples were then incubated with primary antibodies against MDM2 (Proteintech, 1:200), RAGE (Santa, 1:50), STAT2 (Zenbio, 1:100), MMP1 (Affinity, 1:100), COL1A2 (Proteintech, 1:100), and AGEs (Abcam, 1:100) at 4 °C overnight.

Techniques: Immunohistochemical staining, Immunohistochemistry, Staining, Quantitative RT-PCR, Western Blot, TUNEL Assay, Activity Assay, Concentration Assay

Journal: ACS nano

Article Title: Echinacoside-Zinc Nanomaterial Inhibits Skin Glycation by Suppressing the Transcriptional Activation of the Receptor for Advanced Glycation End-Products.

doi: 10.1021/acsnano.3c04726

Figure Lengend Snippet: Figure 3. PPZn exerted antiglycation effects in HaCaT cells. (A) Western blot representative images and quantitative analysis of RAGE, COL1A2, MMP1, AGEs, and β-actin protein levels in HaCaT cells after the indicated treatment. (B) Cell cycle was determined by flow cytometry in HaCaT cells after the indicated treatment. (C) Representative images of TUNEL staining of HaCaT cells after the indicated treatment. Scale bar, 50 μm. (D, E) Relative MDA concentration (D) and SOD activity (E) in HaCaT cells after the indicated treatment. All values are presented as the mean ± SD; P-values determined by two-sided Student’s t test. *P < 0.05, **P < 0.01, compared with the model.

Article Snippet: After being blocked, samples were then incubated with primary antibodies against MDM2 (Proteintech, 1:200), RAGE (Santa, 1:50), STAT2 (Zenbio, 1:100), MMP1 (Affinity, 1:100), COL1A2 (Proteintech, 1:100), and AGEs (Abcam, 1:100) at 4 °C overnight.

Techniques: Western Blot, Flow Cytometry, TUNEL Assay, Staining, Concentration Assay, Activity Assay

Journal: Cytokine

Article Title: IL-33/ST2 enhances MMP-12 expression by macrophages to mediate inflammatory and immune response in IgG4-Related Ophthalmic Disease.

doi: 10.1016/j.cyto.2024.156754

Figure Lengend Snippet: Fig. 2. Expression of IL-33/ST2 in IgG4-ROD A: Immunohistochemistry anal- ysis of IL-33、ST2 in IgG4-ROD lacrimal gland tissues relative to normal tissues. B: Statistical analysis of the immunohistochemistry results from 9 IgG4-ROD patients with lacrimal gland involvement and 9 normal lacrimal gland tis- sues. Normal: lacrimal gland prolapse patients. (*, p < 0.05).

Article Snippet: Antibodies used for immunohistochemical analysis included IL-33 (Proteintech, Catalog no.: 66235–1-Ig), ST2 (Proteintech, Catalog no.: 60112–1-Ig), and MMP-12 (Proteintech, Catalog no.: 66930–1-Ig).

Techniques: Expressing, Immunohistochemistry

Journal: Science advances

Article Title: Selective promotion of sensory innervation-mediated immunoregulation for tissue repair.

doi: 10.1126/sciadv.ads9581

Figure Lengend Snippet: Fig. 8. Sema3A mediates XIAP expression to promote the M2 polarization of macrophages. (A) Schematic representation of macrophage conditioned culture. The super- natant of DRGC culture medium was collected and mixed with the conventional culture medium of macrophages in equal proportion to obtain the CM. (B) Immunofluores- cence staining and (C) quantification of CD206 (M2 marker) and iNOS (M1 marker) expression in macrophages. Scale bar, 30 μm. (D) ELISAs of anti-inflammatory cytokine IL-10 and pro-inflammatory cytokines TNF-α and IL-1β. (E) Immunofluorescence staining and (F) the corresponding quantification of CD206 and iNOS expression in macrophages treated with CM from Sema3A-specific knockdown DRGCs and rSema3A. Scale bars, 30 μm. (G) ELISAs of IL-10, TNF-α, and IL-1β expression after culturing with different CMs. (H) Volcano plot of the differential gene expression in macrophages after Sema3A transfection. (I) Heatmap of the mRNA transcription profiles of the top 15 up-/down- regulated genes. (J) Venn diagram indicating the number of genes overlapping with those collected from the GSEA database (inflammation-related gene sets containing 303 different genes) and the top 15 up-/down-regulated genes screened using RNA-seq. Two genes (Xiap and Mmp13) were overlapping. (K) Representative Western blotting images of XIAP and MMP13 in macrophages after culturing with Sema3A. (L and M) Immunofluorescence images and the corresponding fluorescence intensity of CD206 and iNOS expression in macrophages after shXIAP transfection. Scale bar, 30 μm. (N) ELISAs of IL-10, TNF-α, and IL-1β after shXIAP transfection. (O and P) Immunofluorescence images and the corresponding quantitative analysis of CD206 and iNOS in XIAP-overexpressing macrophages transfected with pcDNA3.1-XIAP. Scale bar, 30 μm. (Q) ELISAs of IL-10, TNF-α, and IL-1β in macrophages overexpressing XIAP. IAN, inferior alveolar nerve; rSema3A, recombinant Sema3A. **P < 0.01; ***P < 0.001; NS, not significant.

Article Snippet: The antibodies used in this part of experiments include Sema3A (1:1000, 27836- 1- AP, Proteintech), MMP13 (1:2000, 181651- AP, Proteintech), XIAP (1:2000, A20846, Abclonal), PAX6 (1:500, A19099, Abclonal), β- actin (1:100000, AC026, Abclonal), Flag- Tag (80010- 1- RR, Proteintech), Myc- Tag (60003- 2- Ig, Proteintech), HA- Tag (#2367, Cell Signaling Technology, USA), and anti- rabbit/mouse- HRP (BA1056, Boster, USA).

Techniques: Expressing, Staining, Marker, Immunofluorescence, Knockdown, Gene Expression, Transfection, RNA Sequencing, Western Blot, Fluorescence, Recombinant

Journal: BMC Genomics

Article Title: Molecular adaptations in MMP genes support lung elasticity and diving adaptations in cetaceans

doi: 10.1186/s12864-025-11751-2

Figure Lengend Snippet: Regression analysis of gene evolution rates with maximum diving depth and time. ( A ) Regression of maximum diving depth against the evolution rate of MMP16 ( R ²=0.2408, P = 0.01186); ( B ) Regression of maximum diving time against the evolution rate of MMP16 ( R² =0.3057, P = 0.00448); ( C ) Regression of maximum diving depth against the evolution rate of MMP9 ( R 2 = 0.3381, P = 0.00268). ( D ) Regression of maximum diving depth against the evolution rate of MMP25 ( R 2 = 0.2026, P = 0.02045); ( E ) Regression of maximum diving depth against the evolution rate of MMP17 ( R 2 = 0.3316, P = 0.00298);( F ) Regression of maximum diving depth against the evolution rate of MMP11 ( R 2 = 0.3120, P = 0.00406)

Article Snippet: The following antibodies were used in the study: Anti- MMP9 (Cat No. #27306-1-AP), Collagen I (Cat No. #14695-1-AP), GAPDH (Cat No. #10494-1-AP), HA (Cat No. #51064-2-AP), and HRP-conjugated Goat Anti-Rabbit IgG (H + L) (Cat No. #SA00001-2), all purchased from Proteintech Group (Wuhan, China).

Techniques:

Journal: BMC Genomics

Article Title: Molecular adaptations in MMP genes support lung elasticity and diving adaptations in cetaceans

doi: 10.1186/s12864-025-11751-2

Figure Lengend Snippet: MMP9 overexpression, protein modifications, and collagen degradation. ( A ) The highest XCorr protein spectrum peak identified from the overexpression of T. truncatus MMP9 . ( B ) In cetaceans, the specific amino acid sites of MMP9 undergo post-translational modifications: the 319 S site is modified with HexNAc, the 532 S site is modified with Phospho and HexNAc, the 661 M site is modified with Oxidation, and the 568 S site is modified with Phospho and HexNAc. ( C ) The highest XCorr protein spectrum peak identified from the overexpression of B. taurus MMP9 . ( D ) Collagen I degradation ability of T. truncatus and B. taurus MMP9 overexpressed in A549 cells. The blots/gels displayed in the figure represent cropped versions. Full-length blots/gels are presented in Fig

Article Snippet: The following antibodies were used in the study: Anti- MMP9 (Cat No. #27306-1-AP), Collagen I (Cat No. #14695-1-AP), GAPDH (Cat No. #10494-1-AP), HA (Cat No. #51064-2-AP), and HRP-conjugated Goat Anti-Rabbit IgG (H + L) (Cat No. #SA00001-2), all purchased from Proteintech Group (Wuhan, China).

Techniques: Over Expression, Modification

Journal: BMC Genomics

Article Title: Molecular adaptations in MMP genes support lung elasticity and diving adaptations in cetaceans

doi: 10.1186/s12864-025-11751-2

Figure Lengend Snippet: Migration ability of A549 cells after MMP9 overexpression. ( A ) Wound healing status after 0–24 h of overexpression of T. truncatus and B. taurus genes. ( B ) Quantitative analysis of wound healing rate, ns: Not significant; *: P < 0.05; **: P < 0.01; ***: P < 0.001; ***: P < 0.0001. P value of 12 h (Tur&Bos; Tur&NC; Bos&NC: 0.0059; 0.0022; 0.0098); P value of 24 h (Tur&Bos; Tur&NC; Bos&NC: 0.0241; 0.001; 0.001)

Article Snippet: The following antibodies were used in the study: Anti- MMP9 (Cat No. #27306-1-AP), Collagen I (Cat No. #14695-1-AP), GAPDH (Cat No. #10494-1-AP), HA (Cat No. #51064-2-AP), and HRP-conjugated Goat Anti-Rabbit IgG (H + L) (Cat No. #SA00001-2), all purchased from Proteintech Group (Wuhan, China).

Techniques: Migration, Over Expression